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名称:高质量、环保LE琼脂糖

品牌:ACTGene 型号:高质量、环保LE琼脂糖

产地:TW 新旧:0

价格:0 数量:2

是否促销:否

浏览次数:30120

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简介:High Quality and Greener LE Agarose


Agarose molecular structure



Affinity column

Applications
1. Gel Electrophoresis
2. Gel plates
3. Gel matrix used in chromatographic separations
4. IEP (Immuno electrophoresis) and Ouchterlony (double diffusion) plates in which antibody-antigen precipitin lines are studied

Feature and Benefit Highlights
‧ High purity and Multi?purpose
‧ Greener agarose choice
‧ Low background and high resolution
‧ Optimized gel strength for ease of gel preparation
‧ Ideal for gel electrophoresis, chromatographic separations, and other life science applications.

QC Analysis
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gelling point - the temperature at which an aqueous agarose solution forms a gel as it cools.
Electroendosmosis (EEO) - one of the most important characteristics of agarose. Anionic group in agarose gel are affixed to the matrix and can not move, but dissociable counter cations can migrate towards the cathode in the matrix, giving rise to EEO, EEO can disrupt separations of DNA because of internal convection. The lower EEO, the faster DNA will migrate, additionally, lower EEO helps to improve the resolution of DNA and RNA as their migration is determined only by their size, not by their charge.

Unique Green Chemistry Manufacturing
Through extensive research and development, we are proud to manufacture our Agarose in an environmentally friendly way by implementing our entire manufacturing process free of any organic solvent use.

Packaging Units : 100g, 500g, 1kg,10kg, 50kg or any size upon request.

Specifications
Product LE Agarose, high purity, Multi?purpose, Low EEO (LE) and sulfate agarose
CAS 9012-36-6
Appearance White to off-white powder
EEO <0.13
Gelling point 36℃±1.5℃(1.5% Gel)
Melting point 88℃±1.5℃(1.5% Gel)
Solubility clear colorless solution at 1g plus 100ml water
Moisture < 10%
Gel strength >1200 g/cm2 (1% Gel)
Sulfate < 0.15%
Ash < 0.5%
Impurities (DNases, RNases, Proteases, and Endonuclease) None detected




Gel Preparation Protocol
1. To make gels with agarose concentration less than 2%:
(1) Use a flask that is 2 to 4 times the volume of the solution being prepared.
(2) Add the correct amount of dry agarose to a measured quantity of electrophoresis buffer.
(3) If use a boiling water bath:
‧ To melt the agarose, simply by heating the slurry in a boiling water bath,bring the solution to a boil and allow it to boil for 5-10 minutes stirring continuously, until the agarose dissolves completely.
If use a microwave oven:
‧ To melt the agarose in solutions of less than 2%, heat the slurry in microwave oven on high power setting until it starts to boil.
‧ Allow the solution to boil for 1 min or until the solution is clear and all particles are dissolved.
‧ Remove the flask from the microwave oven, and gently swirl to mix the agarose solution.
Use caution when handling as solution may be extremely heated.

(4) Cool the solution to approx. 60°C before pouring.


2.To make gels with agarose concentration greater than 2%:
(1) Use a flask that is 2 to 4 times the volume of the solution being prepared.
(2) Add the correct amount of dry agarose to a measured quantity of electrophoresis buffer.
(3) Heat the slurry in a microwave oven on a medium power setting until it starts to boil.
(4) Remove the flask from the oven and gently swirl to resuspend the gel particles.
(5) Reheat the solution on a medium power setting until it starts to boil again.
(6) Afterwards, remove the flask from the microwave and gently swirl.
If the agarose did not completely dissolve, reheat the solution again.
(7) Cool to approx. 70°C before pouring.


Separation of DNA in agarose
Agarose gel (%) 0.3 0.6 0.7 0.9 1.2 1.5 2.0
DNA size range (kb) 60-5 20-1 10-0.8 7-0.5 6-0.4 4-0.2 3-0.1

 

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