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简介：This guide describes Agilent's recommended operational
procedures to analyze DNA copy number variations using
Agilent 60- mer oligonucleotide microarrays for array- based
comparative genomic hybridization (aCGH) analysis. This
protocol is specifically developed and optimized to
enzymatically label DNA from blood, cells or frozen tissues.
For processing FFPE samples, follow the Agilent
Oligonucleotide Array- Based CGH for Genomic DNA
Analysis (ULS Labeling for Blood, Cells, Tissues or FFPE)
Protocol v3.1 (p/n G4410- 90020).
1 Before You Begin
This chapter contains information (such as procedural notes,
safety information, required reagents and equipment) that
you should read and understand before you start an
experiment.
2 DNA Isolation
This chapter describes the method to isolate genomic DNA
(gDNA) from blood, cells, or frozen tissues.
3 Sample Preparation
This chapter describes the two methods to process genomic
DNA (gDNA) prior to labeling.
4 Sample Labeling
This chapter describes the steps to differentially label the
gDNA samples with fluorescent- labeled nucleotides.
5 Microarray Processing and Feature Extraction
This chapter describes the steps to hybridize, wash and scan
Agilent CGH microarrays and to extract data using the
Agilent Feature Extraction Software. |
